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. 2019 Jun 7;6(3):ENEURO.0066-19.2019. doi: 10.1523/ENEURO.0066-19.2019

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Copyright © 2019 Bimpisidis et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 7. — Optogenetic stimulation in striatal target areas of NeuroD6 and Calb2 VTA neurons reveals glutamatergic postsynaptic responses. A, Representative picture from patch-clamp slice electrophysiology in NAcSh of NEX-Cre mice and OT of Calb2-Cre mice injected with DIO-ChR2-eYFP. B, Representative traces of photostimulation-evoked postsynaptic currents recorded from NAcSh cells from NEX-Cre and OT cells from Calb2-Cre mice injected with DIO-ChR2-eYFP. C, Pie charts represent the percentage of cells showing EPSCs (white) versus negative (black) upon photostimulation of terminals in the NAcSh (N = 18 cells from four mice) of NEX-Cre mice, and OT (N = 14 cells from 4 mice) of Calb2-Cre mice. The y-axis shows amplitude in pA; each circle represents one cell, and bold lines the mean amplitude ± SEM. D, Patch-clamp recordings pre-bath (control) and post-bath application of DNQX upon photostimulation in NAcSh of NEX-Cre/ChR2 (left, N = 6 cells from three mice) and OT of Calb2-Cre/ChR2 mice (right, N = 5 cells from three mice). Each circle represents one cell (*p < 0.05 control vs DNQX). NAcSh, nucleus accumbens shell; OT, olfactory tubercle. Calb2, Calbindin 2 (Calretinin); NEX; NeuroD6; ChR2; Channelrhodopsin 2; eYFP, enhanced Yellow fluorescent protein.