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. 2019 Jun 7;6(3):ENEURO.0066-19.2019. doi: 10.1523/ENEURO.0066-19.2019

Figure 8.

Figure 8.

Optogenetic activation of NeuroD6 VTA neurons, but not Calb2 VTA neurons, induces place preference. A, Schematic drawing of stereotaxic injection into VTA of Cre-dependent DIO-ChR2-eYFP and of experimental setup for RT-PP analysis. B–F, Time spent in light-paired (blue), unpaired (white during phase 1, black during reversal phase), and neutral (gray) compartments shown as mean percentage of time spent in each compartment ± SEM (left; *p < 0.05, ***p < 0.001 paired vs unpaired compartment); average percentage of time spent in each compartment during days 3, 4, 6, and 7 ± SEM (bar graphs; right; *p < 0.05, ***p < 0.001 vs light-paired compartment; #p < 0.05, ##p < 0.01, ###p < 0.001 vs unpaired compartment). DAT-Cre N = 10; Vglut2-Cre N = 7; Calb2-Cre N = 7; NEX-Cre N = 5. F, High-power stimulation of bilaterally injected NEX-Cre mice (N = 4). G, Schematic illustration of optical fiber placement in mice analyzed in RT-PP analysis. NS, non-significant. DAT, Dopamine transporter; Calb2, Calbindin 2 (Calretinin); NEX, NeuroD6; Vglut2; Vesicular glutamate transporter 2; ChR2; Channelrhodopsin 2; eYFP, enhanced Yellow fluorescent protein.