a. A diagram of sphingolipid structure. R indicates the substituent of sphingolipid. The FA chain has various carbon number while the LCB moiety are largely synthesized as saturated form (18:0) and desaturated form (18:1) with different OH (d/t) number. b. Simplified schematic representation of sphingolipid biosynthesis pathway in plants. c and d. Free LCB(P), ceramide and complex sphingolipid species characterized by LCB(d18:0, d18:1, t18:0, t18:1) and FA(16:0-26:1) pairings in wild-type C24 and plm-1(c) and Col-0 and plm-2 (d). 3-KS, 3-ketosphinganine. e.Total levels of complex sphingolipid species in wild-type and plm mutants. Measurements are the average of five replicates (n = 5 independent samples). Data are means ± SD.Statistically significant differences are indicated (two-tailed Student’s t-test; *P<0.05. ** P <0.01).