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. Author manuscript; available in PMC: 2019 Aug 11.
Published in final edited form as: Oncogene. 2019 Feb 11;38(24):4700–4714. doi: 10.1038/s41388-019-0745-2

Figure 5. Inhibition of KPNB1 using importazole induces apoptosis and suppresses sphere formation of PCa through NF-κB signaling.

Figure 5.

A) Immunoblotting results showing nuclear NF-κB p50 of PC3 or C42B that was transfected with KPNB1 siRNA (50 nM) or control siRNA for 48 hours. Lamin A/C was used as internal control. B and C) Immunoblotting results showing nuclear NF-κB p50 of PC3 or C42B that was treated with indicated concentrations of importazole or DMSO. Lamin A/C was used as internal control. D and E) Flow cytometry showing the apoptosis of PC3 or C42B that was transfected KPNB1 siRNA (50 nM) or control siRNA for 48 hours. Annexin V positive cells were counted as apoptotic population. F and G) Flow cytometry showing the apoptosis of PC3 or C42B that was treated with indicated concentrations of importazole or DMSO. Annexin V positive cells were counted as apoptotic population. Sphere formation assay of PC3 or C42B that was H) treated with indicated concentrations of importazole or DMSO, or I) transfected with KPNB1 siRNA (50 nM) or control siRNA for 1 week. *p <0.05, **p<0.01, ****p<0.0001.