Table 1.
Parameters from in-cell and in vitro calibrations, using 2p-excitation.
| calibration | Temp. (°C) | LTmin ± SD (ns) | LTmax ± SD (ns) | K0.5 ± SD (mM) | nH |
|---|---|---|---|---|---|
| In-cell | 34 | 1.403 ± 0.009 | 1.784 ± 0.012 | 1.46 ± 0.50 | 1 |
| 37 | 1.370 ± 0.010 | 1.752 ± 0.020 | 1.79 ± 0.32 | 1 | |
| In vitro | 34 | 1.399 ± 0.023 | 1.754 ± 0.035 | 2.07 ± 0.78 | 1 |
| 37 | 1.354 ± 0.041 | 1.697 ± 0.040 | 2.17 ± 0.60 | 1 |
*
The Hill coefficient (nH) was set to 1 in the fits. Values for the other parameters are expressed as the mean ± SD of three independent dose-response curves.