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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: J Neurosci Res. 2019 Feb 23;97(8):975–990. doi: 10.1002/jnr.24397

Figure 5.

Figure 5

SIRT3 is required for NMN induced decrease in mitochondrial protein acetylation. Hippocampal tissue was isolated from NMN (62.5 mg/kg) or vehicle (PBS) treated animals. SIRT3 target, SOD2, was immunoprecipitated from hippocampal tissue samples and acetylation levels were analyzed by western blot. (A) NMN induced a 30% decrease in acetylation of SOD2 compared to vehicle treated animals. (B) In samples from SIRT3KO mice NMN did not affect mitochondrial protein acetylation. *p<0.05, ***p<0.001 compared to vehicle (n=7 (A), n=12 (B)) (A Student T-test, B One-way ANOVA followed by Tukey HSD test)