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. Author manuscript; available in PMC: 2019 Aug 26.
Published in final edited form as: Oncogene. 2019 Feb 26;38(24):4820–4834. doi: 10.1038/s41388-019-0760-3

Figure 1. DNA copy numbers for chromosome 8q24.21 in human prostate cancer cells.

Figure 1.

(a) Diagram of the position of human miRs-1204~1208, coding gene c-MYC, and long non-coding RNA PVT1 at 8q24.21 and a reference locus at 8q22. Down-arrows indicate loci of miRs-1204~1208. Horizontal arrows indicate the loci for design of PCR primers. (b) Relative DNA copy number of 8q24.21 and 8p22 loci against multiple independent loci in the genome determined by a multicopy reference assay of human prostate cancer cell lines. Data are presented as means ± SD. * p < 0.05 by two-tailed t-test for PCR primers 1–3 vs. primer 4. (c) Representative images of laser capture microdissection of tumor cells in prostate cancer tissues. Left panels: H&E staining; Right and middle panels: laser capture microdissection of tumor cells from target tissues. (d) Relative DNA copy numbers of 8q24.21 and 8p22 loci in primary castration-resistant prostate cancer specimens. Data are presented as the means ± SD. * p < 0.05 by two-tailed t-test in T group vs. N group. T, micro-dissected prostate cancer cells; N, micro-dissected normal prostate epithelial cells. All experiments were repeated three times.