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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Proteomics Clin Appl. 2018 Dec 19;13(4):e1800129. doi: 10.1002/prca.201800129

Figure 2. Flow Diagram of Affinity Chromatrography.

Figure 2.

Columns were loaded with serum from patients who had received either a mechanical or GFBP valve. IgG antibodies were captured within the column and non-IgG serum components were washed off the column. The IgG antibodies were crosslinked to the column and then bovine pericardial protein extract was added. Non-specific proteins were washed off the column while captured proteins were eluted separately and submitted for LC-MS/MS analysis. Differences between the two groups were analyzed to determine which proteins were antigenic.