Figure 1.

Visualization through agarose gel electrophoresis of the fragments amplified with the different pairs of primers used. The two pairs of primers described by Kosaki et al. (1997) amplify both methylated and nonmethylated allele‐producing bands with 174 bp and 131 bp respectively, being possible to distinguish abnormal methylation patterns. The unique pair of primer designed and proposed by this study amplifies both alleles equally generating a single fragment band with 196 bp. The single band of 196 bp is due to the designed primer characteristic of annealing outside of the CpG island in SNURF‐SNRPN locus. The allelic discrimination using the designed primer proposed by our study is possible through techniques such as HRM