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. 2019 Mar 29;7(6):e670. doi: 10.1002/mgg3.670

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© 2019 The Authors. Molecular Genetics & Genomic Medicine published by Wiley Periodicals, Inc.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Immunohistochemistry and immunofluorescent staining of Cx43. (a, b) In control healthy skin, immunosignals of anti‐Cx43 were expressed in the intracellular junctions of keratinocytes in the stratum corneum and granular layer (×100, DAB stain, ×200). (c) In lesion of patient 1, immunosignals of anti‐Cx43 were strongly expressed in the stratum corneum, granular layer and spinous layer, whereas basal layer show low expression of Cx43 (×100, DAB stain). (d) Cx43 protein was mainly expressed in the cytomembrane and cytoplasm (×200). (e) In lesion of patient 2, immunosignals of anti‐Cx43 were strongly expressed in the stratum corneum and granular layer (×100, DAB stain). (f) Cx43 protein was mainly expressed in the cytomembrane and cytoplasm (×200)