Figure 3.
miR‐1281 was a target gene of GIHCG. (a) Bioinformatic analysis showing their potential binding site. (b,c) Dual luciferase reporter gene assay demonstrating their binding relationship. (d) Significant lower miR‐1281 expression after upregulation of GIHCG expression in HGC‐27 and SGC‐7901 cells; E Significantly low miR‐1281 expression in gastric cancer tissues relative to adjacent tissues. (f) Negative correlation between GIHCG and miR‐1281 in gastric cancer, p < 0.05, R 2 = 0.7192. (g) After upregulation of miR‐1281 expression in HGC‐27 and SGC‐7901 cells, significant inhibition of cell proliferation and partial reversion of the promotion effect of GIHCG on cell proliferation. (h) After upregulation of miR‐1281 expression in HGC‐27 and SGC‐7901 cells, significant inhibition of cell migration and partial reversion of the promotion effect of GIHCG on cell migration. *p < 0.05, **p < 0.01, ***p < 0.001