Figure 3.

NEAT1 directly interacts with miR‐34a‐5p. (a) Cytoplasmic and nuclear levels of NEAT1 in HOS and Saos2 cells analyzed by qRT‐PCR. (b) MiR‐34a‐5p expression in osteosarcoma tissues. (c) Bioinformatics evidence of binding of miR‐34a‐5p onto 3′‐UTR of NEAT1. (d) Dual‐luciferase reporter gene assay in HOS and Saos2 cells after transfection with negative control or miR‐34a‐5p mimics, renilla luciferase vector pRL‐SV40 and the reporter constructs. (e) RIP experiments for the amount of NEAT1 and miR‐34a‐5p in HOS and Saos2 cells. Data are presented as mean ± SD. *p < 0.05