Skip to main content
. 2019 Jun 4;26:101238. doi: 10.1016/j.redox.2019.101238

Fig. 2.

Fig. 2

L-arginine analogues containing a guanidino nitro group potentiate vasodilation by BDNIC and nitroglycerin (NTG). Rats were given L-NAME, D-NAME, L-Arg, or L-NMMA via i.p. injection for 4 days at 222 μmol·kg-1·day-1; another group was given L-NAME by oral gavage (p.o.) at the same dose. Blood pressure and flow responses in the mesenteric artery were then measured in response to nitrodilators. Vasodilation by A) BDNIC and B) NTG was potentiated by L-NAME and D-NAME, which contain a nitro group, but not by L-arginine and L-NMMA, which do not. A nitro group present in L-NAME and D-NAME but absent in L-arginine and L-NMMA potentiates dilation caused by A) BDNIC and B) NTG. Two-way ANOVA, n ≥ 5. C-E) Effects of different L-arginine analogues on C) mean arterial blood pressure (MAP), D) mesenteric arterial conductance, and E) plasma nitrite concentration under baseline conditions. One-way ANOVA, n ≥ 5. F) L-NAME pretreatment in rats increases baseline mesenteric arterial tissue NO metabolites (NOx) concentration. t test, n=4. G) Effects of different L-arginine analogues on bradykinin (eNOS-dependent) mediated relaxation. One mM of each guanidine was applied 15 min before contraction. t test, n ≥ 4. H) i.p. injection of a single dose of L-NMMA (222 μmol·kg-1) increased MAP in intact rats. Paired t test, n=3. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. Control.