Skip to main content
. 2019 May 9;12(6):1329–1341. doi: 10.1016/j.stemcr.2019.04.009

Figure 3.

Figure 3

mESC-Derived Oculomotor Neurons Have High Levels of Akt Signaling

(A) RPKM values from RNA sequencing of mESC-derived oculomotor and spinal motor neurons enriched by FACS shows preferential expression of Akt1 and Akt3 transcripts in the generated OMNs (bar and whiskers represent means ± SEM, ∗adjusted p < 0.05, n = 6).

(B and C) Microphotographs show pAKT levels in OMN (B) and SC MN (C) cultures at D7 toxicity assay assessed by immunohistochemistry, pAKT staining alone in small insets (red channel).

(D and F) Fluorescence intensities (D), analyzed in a semi-quantitative manner, indicate higher expression of pAKT in OMNs (F) (bar and whiskers represent means ± SEM, two-way ANOVA and Tukey's multiple comparison test, F(1, 353) = 66.1, ∗∗∗p < 0.0001, n = 4). Scale bar in (F), 60 μm (applies also to B and C).

(E and G) β-CATENIN expression is maintained in OMN also during kainate toxicity (E). (G) Semi-quantitative analysis of β-CATENIN over NF200 staining intensities in OMNs and SC MNs in kainate conditions (bar and whiskers represent means ± SEM, t test, t = 6.799, df = 69, ∗∗∗p < 0.0001, n = 3). Scale bar in (E), 60 μm.