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. 2019 Jun 13;9:8608. doi: 10.1038/s41598-019-44887-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Glycerol supplementation suppresses the potency of Q203 on agar plates. M. tuberculosis H37Rv was plated on agar plates at a density of 1 × 10⁶ bacilli on 7H10-OADC plates supplemented with glycerol (0.5%) or pyruvate (20 mM) and containing no antibiotics (A, B: left quadrants), 100 nM of Q203 (A, right quadrants), or 500 nM of BDQ (B, right quadrants). Pictures were taken after 15 days of incubation at 37 °C.