Table 1.
Glycerol and Q203 treatment triggers an upregulation of the cydAB operon in mycobacteria.
| Genes | Expression level (Fold change) | |||
|---|---|---|---|---|
| −Glycerol | +Glycerol | |||
| − | +Q203 | − | +Q203 | |
| cydA | 1.0 | 2.7 ± 0.7 | 2.2 ± 0.1 | 8.1 ± 0.2 |
| cydB | 1.0 | 2.9 ± 0.4 | 2.2 ± 0.2 | 7.4 ± 0.3 |
| qcrC | 1.0 | 0.8 ± 0.4 | 0.5 ± 0.1 | 1.4 ± 0.4 |
| qcrA | 1.0 | 0.75 ± 0.4 | 0.47 ± 0.1 | 1.01 ± 0.5 |
| qcrB | 1.0 | 0.90 ± 0.3 | 0.50 ± 0.1 | 1.31 ± 0.6 |
Expression levels were measured by qRT-PCR. The gene sigA was used as an endogenous control (housekeeping gene) to determine the relative expression of the M. bovis qcrCAB and cydAB operons in culture broth media supplemented with glycerol and/or Q203.