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. 2019 Jun 13;9:8594. doi: 10.1038/s41598-019-45083-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Efficient recovery of long capped RNA transcripts by a decapping/recapping procedure. A mixture of m⁷G-capped RNAs (0.090–1.4 kb) was divided into two samples. One sample was incubated with yDcpS and one without yDcpS, and both were subsequently treated with VCE and DTB-GTP. An equal fraction of each was exposed to streptavidin beads, which were washed and eluted. Lanes 1 (+yDcpS) and 2 (−yDcpS) show the samples after the VCE reaction. Lanes 3 (+yDcpS) and 4 (−yDcpS) show the eluates from streptavidin beads. All lanes represent an equal fraction of the original mixture. Both gel panels are from the same imaged gel.