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. 2019 Jun 13;10:2593. doi: 10.1038/s41467-019-10554-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Molecular requirements of PLD function in axonal localization. a Primary sequences of the wild-type (top) and scrambled (bottom) PLDs. Scramble variants were generated randomly and exhibit different degree of Glutamine dispersion. b–e Medial lobes of adult brains expressing GFP-Imp (b), GFP-Imp-Scr2 (c), GFP-Imp-Scr4 (d), or GFP-Imp-Nter-PLD (e) under the control of the 201Y-Gal4 driver. Genotypes: 201Y-Gal4/UAS-GFP-Imp or UAS-GFP-Imp-Scr2 or UAS-GFP-Imp-Scr4 or UAS-GFP-Imp-Nter-PLD. Scale bar in b–e: 15 μm. f, g Distributions of normalized GFP signal intensities in distal axons. Box plots are represented using the min to max convention, where the middle line defines the median and the whiskers go down to the smallest value and up to the largest. Numbers of MB analyzed: UAS-GFP-Imp: 8 (for f, g); UAS-GFP-Imp-Scr2: 6; UAS-GFP-Imp-Scr4: 8; UAS-GFP-Imp-Nter-PLD:9. ns stands for not significant (Mann–Whitney test). Source data are provided as a Source Data file