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. 2019 Jun 13;10(6):465. doi: 10.1038/s41419-019-1712-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a The spheroid formation assay was performed to assess the effect of AQP3 on the self-renewal capacity of CD133+ HCC cells. Results showed AQP3 knockdown significantly reduced the number of spheroids (**P < 0.01) and AQP3 overexpression increased the number of spheroids (*P < 0.05). b The Hoechst staining and flow cytometry sorting were used to test the side population proportion in CD133+ HCCLM3 and HUH7 cells. Results showed AQP3 depletion significantly reduced the SP proportion (**P < 0.01) and AQP3 overexpression increased the SP proportion (*P < 0.05, **P < 0.01)