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. 2019 Jun 13;10(6):465. doi: 10.1038/s41419-019-1712-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a The CD133+ cell population ratio in HCCLM3 and HUH7 cells was assessed using FACS methods. Results showed AQP3 overexpression could increase the ratio of CD133+ cell population (**P < 0.01). AG-490 could decrease the CD133 + cell proportion (**P < 0.01) and eliminate the influence of AQP3 overexpression (**P < 0.01). b AQP3 overexpression could up regulated CD133 mRNA and protein expression in HCC cells (**P < 0.01). AG-490 downregulated CD133 expression (**P < 0.01) and eliminated the influence of AQP3 overexpression (**P < 0.01). c, d AQP3 overexpression could enhanced the stemness of CD133 + HCC cells (*P < 0.05, **P < 0.01). AG-490 restrained the stemness of CD133 + HCC cells (**P < 0.01) and eliminated the influence of AQP3 overexpression (**P < 0.01). e, f CHIP assays was used to detect the CD133 promoter (p-CD133) acetylated histone H3 levels in HCCLM3 and HUH7 cells. Results showed AQP3 knockdown decreased p-CD133 acetylated histone H3 levels (**P < 0.01). g, h AQP3 overexpression increased p-CD133 acetylated histone H3 levels (**P < 0.01). AG-490 decreased p-CD133 acetylated histone H3 levels (**P < 0.01) and eliminated the influence of AQP3 overexpression (**P < 0.01)