Fig. 2.

Physical barrier functions of the human BBB reconstituted on-chip. a Graph showing protein expression ratios of ABC and SLC transporter proteins in the iPS-hBMVECs differentiated under hypoxic conditions relative to normoxic conditions. b Impedance values for the human BBB measured in the TEER chip over the first 7 days of culture on-chip (recorded in the frequency range of 0.1 Hz–100 kHz) with iPS-BMVECs that were differentiated under hypoxia (closed circles) or normoxia (open triangles). c Impedance values for the BBB formed by the same human astrocytes, pericytes, and iPS-hBMVECs that were differentiated under hypoxia (closed circles) or normoxia (open triangles) but cultured in static Transwell inserts measured by TEER measurement machine (EVOM2). Data are represented as ± SE, N = 3. Statistical analysis is two-way ANOVA with Tukey’s multiple comparisons test; *P < 0.05; **P < 0.001; ***P < 0.0001