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. 2019 Jun 13;9:8620. doi: 10.1038/s41598-019-45134-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of dilution factor, time changes and standard albumin concentration on oxidized albumin%. (a) Dilution test. We diluted serum samples with PBS. The minimal concentration that can be measured by our method is 6.4 mg/ml in serum. (b) Time-dependent oxidation of albumin. Samples were left at room temperature before analysis. The horizontal axis indicates the time that the sample was left at room temperature (0 min, 20 min, 40 min, 60 min, 80 min, 100 min, or 120 min). The vertical axis indicates the percentage of oxidized albumin of each sample. The samples used were from healthy rats. (c) Standard albumin/total albumin% dependence on oxidation of albumin. Standard albumin was added in 8% increments, and oxidized albumin% was increased linearly.