Figure 11.

The rate of endolysosomal and phagosomal fusion was increased in lvsB-null cells. A enumerates the number of red/green-merged endosomal vesicles and reveals that this number is significantly higher in the lvsB-null cells relative to control (wt) and lvsA-null cells (unpaired, two-tailed Student's t test, p = 0.0016). B illustrates that the number of fusion events per cell is significantly higher at the 30-min chase point in the lvsB-null mutant as compared with lvsA-null and control cells (wt) when using FITC-labeled bacteria (see MATERIALS AND METHODS for details) as a marker for phagolysosomes (unpaired, two-tailed Student's t test, p < 0.0001). These experiments were repeated three times each and for each experiment between 50 and 100 cells in random fields were analyzed.