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The enlarged vesicles in the lvsB-null mutant are endocytic. NC4A2 control (A and B), lvsA-null (C and D), and lvsB-null (E and F) cells were incubated in HL-5 medium containing FITC-dextran for 1 h to illuminate the endocytic vesicles. Cells were visualized using a fluorescence microscope. The arrows point to enlarged endocytic vesicles in lvsB-null cells, and the arrowheads point to vesicles the approximate size of normal lysosomes. Bar, 1 μm.
