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. 2019 Jun 13;9:8609. doi: 10.1038/s41598-019-44950-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GD3 expression is increased with SIAE induction in RES256 cells. (A) Using flow cytometry expression of GD3^A was found to be significantly reduced in EGFP expressing controls (p < 0.0180). No difference was seen in SIAE clones 1, 2 or S127A. (B) GD3 expression was significantly increased in SIAE clone 2 (p = 0.0171). No significant changes are seen in SIAE 1, S127A or EGFP expressing cells. SIAE expression was induced for 48 hours with doxycycline. Samples were analysed using a multi-parameter fluorescence-activated cell sorting (FACS) Calibur flow cytometer and CellQuest Pro software. Data analysed by one-way analysis of variance followed by Tukey’s multiple comparisons post-hoc test using Graph Pad (^*p < 0.05; ^**p < 0.01) n = 3 ± SEM.