Fig. 5.

TssE and VasX contribute to the regulation of T6SS assembly. a The sheath assembly was monitored by TssB-msfGFP localization in the absence of TssE, VasX, or both. Representative images of GFP channel are shown for each indicated strain (all tssB-msfGFP background). The upper panel shows the cells grown to OD₆₀₀ 0.6; the lower panel shows the cells grown to OD₆₀₀ 1.5. The average number of sheaths is indicated below each image. Time-lapse movies with a larger field of view can be found in Supplementary Movies 3 and 4. b The sheath assembly was monitored by TssB-msfGFP localization with either tssE or vasX expressed under arabinose promoter in tssB-msfGFP strains lacking both tssE and vasX. The average number of sheaths is indicated below each image. Scale bar: 5 µm. Time-lapse movies with a larger field of view can be found in Supplementary Movie 5. Source data are provided as a Source Data file