Fig. 6. DIM mediated abrogation of CPT-induced carcinogenesis and EMT.

a HCT-116 cells seeded onto FITC-gelatin coated coverslips were treated with Vehicle, 250 nM CPT, 250 nM CPT + 25 μM DIM, and 25 μM DIM for 36 h and the slides were observed in fluorescence microscope at ×20 magnification. b Hematoxylin and Eosin staining of intestinal tissue obtained from induced AhCre-ErT Apcfl/fl treated with Vehicle, 0.4 mg/kg CPT, 0.4 mg/kg + 20 mg/kg DIM, and 20 mg/kg DIM for 48 h. Images were taken at ×20 magnification. c Immunohistochemistry analysis of Vimentin and p^ser38 Vimentin was performed on intestinal tissues obtained from AhCre-ErT Apcfl/fl mice treated with Vehicle, 0.4 mg/kg CPT, 0.4 mg/kg + 20 mg/kg DIM, and 20 mg/kg DIM for 48 h. Images were acquired in ×20 magnification