Table 4.
Functional annotation clustering.
| Functional Annotation Clustering | ||||
|---|---|---|---|---|
| Group cluster | Gene name | Accession | Regulation | Description |
| Ribosomal proteins (ES 1.7)* | ||||
| Hnrnpr | Q566E4 | Down | Heterogeneous nuclear ribonucleoprotein R | |
| Mrpl11 | A0A0G2JYU2 | Down | 39S ribosomal protein L11, mitochondrial | |
| Mrpl28 | D3ZJY1 | Up | 39S ribosomal protein L28, mitochondrial | |
| Mrpl44 | Q4G067 | Down | 39S ribosomal protein L44, mitochondrial | |
| Mrps5 | D3ZYT2 | Down | 28S ribosomal protein S5, mitochondrial | |
| Mrps16 | D4A7X1 | Down | 28S ribosomal protein S16, mitochondrial | |
| Mrps30 | D4A833 | Down | 28S ribosomal protein S30, mitochondrial | |
| Mrps34 | D4ABM5 | Down | 28S ribosomal protein S34, mitochondrial | |
| Nop58 | Q5PPK6 | Down | Nucleolar protein 58 | |
| Rpl22l1 | B2RZD5 | Up | 60S ribosomal protein L22-like 1 | |
| Rps5 | B0BN81 | Up | 40S ribosomal protein S5 | |
| Mitochondrial transit peptides (ES 1.6)** | ||||
| Ccbl1 | G3V827 | Down | Cysteine conjugate-beta lyase 1, isoform CRA_a | |
| Cox7a2 | B2RYS0 | Down | Cytochrome c oxidase subunit 7A2, mitochondrial | |
| Dlst | G3V6P2 | Down | Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial | |
| Micu1*** | A0A0G2K0T5 | Down | Calcium uptake protein 1, mitochondrial | |
| Mrpl11 | A0A0G2JYU2 | Down | 39S ribosomal protein L11, mitochondrial | |
| Pdp2 | A0A0G2JSL7 | Down | [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2, mitochondrial | |
| Prdx3 | G3V7I0 | Down | Thioredoxin-dependent peroxide reductase, mitochondrial | |
Functional annotation clustering was performed using the bioinformatics software DAVID (https://david-d.ncifcrf.gov/). Proteins with ≥3 scans, ≥1 unique peptide, fold change >1.136 or <0.88, and a p-value < 0.05 were included and analyzed together, or separated into down-regulated and up-regulated proteins. Two annotation clusters were found with an ES ≥1.3. *ES of 1.7 when interrogating the complete list of proteins. This cluster was also found when interrogating only down-regulated proteins (ES 1.3). **ES of 1.6 when investigating only down-regulated proteins. ***MICU1 is not classified as a transit peptide, but was within the cluster group as a mitochondrial protein. Gene names are listed, together with accession number and a short description of each gene and cluster. The description of genes was accessed from uni-prot.org, using the descriptions from the human database, when not available for Rattus norvegicus. ES: enrichment score.