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. 2019 Jun 13;2:205. doi: 10.1038/s42003-019-0453-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Mononuclear tetraploid content distinguishes rodent cardiac stem cells in vitro. G-band karyotype analysis performed upon cultured CSCs reveals tetraploid content of rodent mouse (a) and rat (b) in contrast to diploid content of swine (c) and human (d) samples. Immunocytochemistry of CSC verifies mononuclear content of karyotyped cells (scalebar = 500 μm) (e–h) and zoomed-in images (scalebar = 100 μm) (e′–h′). Multiple samples from different CSC lines verify consistent tetraploid content in adult mouse (i) and rat (j) CSCs, while swine (k) and human (l) CSCs are consistently diploid. Cell counts included in flow-cytometry analysis provided in parenthesis