Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jun 13;2:205. doi: 10.1038/s42003-019-0453-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — Distinct population characteristics of diploid versus tetraploid fresh murine Lin–c-kit+ isolates revealed by single-cell RNA sequencing (scRNA-Seq). Freshly isolated, viable diploid and tetraploid Lin–ckit+ CICs from adult FVB mouse were analyzed using scRNA-Seq. The diploid population (salmon) predominately cluster together, while tetraploid cCICs (teal) cluster in a different cell group (a; cells analyzed per group identified next to ploidy state). Identification of the cell populations demonstrates that cCICs are a heterogeneous population of fibroblast, endothelial, and lymphocyte cells (b). Percent of cCICs based on cell type (c). Percent of each cell type based on ploidy content (d). Heatmap of upregulated differentially expressed genes (DEGs) specific to endothelial and fibroblast markers confirm transcriptional differences in murine-derived diploid (2N_F) and tetraploid populations (4N_F) (e). These DEGs are also displayed in frequency and expression level between the diploid and tetraploid cCICs (f). The top ten gene ontology (GO) terms upregulated in the 2N population display extracellular matrix cellular components, while the 4N population cellular component is junction oriented (g). The top ten GO terms by biological process upregulated in the 2N population represent both extracellular matrix and angiogenesis processes, while the 4N population is primarily angiogenesis oriented (h)