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. 2002 Feb;13(2):670–682. doi: 10.1091/mbc.01-10-0513

Figure 6.

Figure 6

The effect of ART-27 on AR transcriptional activation is dependent on the AR-interacting region. (A) HeLa cells were transfected with 0.5 μg of the pcDNA3LexA:AR N-terminal derivatives indicated and either 1.0 μg of the empty expression vector (white bars) or 1.0 μg pcDNA3-HA–ART-27 (gray bars) along with 1.0 μg of the LexA responsive-luciferase reporter (pΔ4X-LALO) and 0.25 μg of pCMV-LacZ, and AR activity was determined as described in Figure 5. Data represent the means of duplicate data points normalized to β-galactosidase units. Error bars represent the range of the duplicate data points. (B) The expression of the LexA:AR derivatives was analyzed by immunoblotting from a parallel set of transfections using a polyclonal antibody to LexA.