Figure 3.

Lympho-Organoids Are Connected to Lymphatics and Promote Lymphatic Perfusion

(A) Scheme of pre-LO transplantation at the site of popliteal dissected LNs.

(B) Representative fluorescence images of LO surrounded and infiltrated by FITC⁺PROX-1⁺ lymphatics (orange). Insets ii and iii are magnifications from panel i. AV, afferent vessel; EV, efferent vessel, LO, lympho-organoid. Scale bars: 500 μm (panel i) and 200 μm (ii and iii). Images are representative of two independent experiments.

(C) Representative FITC and NIR fluorescence images of lymphatic vasculature showing connections between LOs and saLN through EV. EV, efferent vessel; LO, lympho-organoid; saLN, sacral LN. Images are representative of two independent experiments. Scale bars: 1,000 μm.

(D) Quantification analysis of lymphatic perfusion. Data presented as pooled number of mice used from two independent experiments. ^∗∗∗p < 0.001.