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. 2019 Jun 13;10:2595. doi: 10.1038/s41467-019-10600-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Follow-up evolution experiment of evolved pCON hosts at 37 °C. a Plasmid persistence under non-selective conditions after selection for evolved plasmid-carrying populations (green) compared against pCON persistence during the first experiment (Fig. 1a) at 37 °C (red, dashed lines). The experiment was conducted for 56 transfers corresponding to about 340 generations in L populations, 450 generations in M populations, and 560 generations in S populations. b Sequencing coverage distribution of ancestral and evolved pCON variants from population S6. The presented coverage was divided by the mean coverage. c Genomic map of pCON plasmids. The pCON variant S6 encountered a segmental duplication downstream the nptII gene including 125 bp of nptII and 372 bp of the oriV. The fusion site is indicated by a star symbol. In pCON-ran a random DNA segment of 500 bp was placed between nptII and the oriV. d Nucleotide sequence of the fusion site of nptII and the oriV. The black box indicates a microhomology region (8 bp) within which the illegitimate fusion potentially occurred. Extended homologies are shown up and downstream of the fusion site. Source data are provided as a Source Data file