Figure 2.

Functional Comparison of hiPSC-Mono and Blood-Mono in the Microfluidic Adhesion Assay

(A) FACS analysis of surface expression of CD14 and CD45 on hiPSC-mono and Blood-mono after cryopreservation. Error bars are ±SD of three independent experiments. Unpaired t test: ns, non-significant.

(B) Schematic for the microfluidic flow adhesion assay of monocytes and ECs.

(C) Representative images taken at the end of the flow assay for each combination of ECs and monocytes. Monocytes were labeled with DiOC6 (green). Scale bar represents 200 μm.

(D) Quantification of the number of adhered monocytes: hiPSC-mono and hiPSC-ECs, Blood-mono and hiPSC-ECs, hiPSC-mono and HUVECs, Blood-mono and HUVECs. Error bars are ±SD of four independent experiments. Uncorrected Fisher's least significant differences test: ns, non-significant; ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001.

(E) FACS analysis of surface expression of MAC-1 (CD11b and CD18) and VLA-4 (CD49d and CD29) integrin subunits on hiPSC-mono and Blood-mono. Error bars are ±SD of three independent experiments. Unpaired t test: ns, non-significant; ^∗∗p < 0.01, ^∗∗∗p < 0.001.

(F) FACS analysis of ICAM-1, E-Selectin, VCAM-1, VE-cadherin, CD31, and CD105 on hiPSC-ECs and HUVECs after TNF-α treatment. Isotype control is shown in red and antigen-specific antibody is shown in blue.

See also Figure S2.