Table 1.

Examples of misfolding proteins involved in neurodegenerative diseases that undergo cell-to-cell transfer in different conditioned medium experimental set ups. N.A., not applicable, N.C., not characterised.

Experimental set up	Protein of interest	Disease	Transferring species	Cell line
Membrane barrier	Dipeptide repeat proteins from C9orf72 hexanucleotide repeat expansions	ALS	N.C.	NSC-34 [51]
	SOD1	ALS	N.C.	Neuro 2 A [45]
Application onto acceptor culture	Tau	Alzheimer's	N.C [80]., Fibrils [81]	HEK293 [80], Primary neurons [81]
	Huntingtin (Q19 and Q103)	Huntington's	N.C.	HEK donors and SH-SY5Y acceptors [82]
	α-Synuclein	Parkinson's	Monomers, oligomers and fibrils [83] High and low molecular weight oligomers [84]	SH-SY5Y donors and COS-7 acceptors [83] Differentiated and undifferentiated SH-SY5Y [84]
	SOD1	ALS	N.C [45].	Neuro 2 A [45]
	TDP-43	ALS	N.C.	HEK293 [27], [74]
Exosome isolation	Yeast prion Sup35 (NM domain)	N.A.	Multimers [85] N.C [86].	Neuro2A [85], [86]
	SOD1	ALS	N.C.	NSC-34 [66]
Complementary protein assay model	Tau	Alzheimer's	N.C.	HEK293T [87]
	TDP-43	ALS	Dimers, oligomers	HEK293 [75]