Figure 1.

Age-Dependent Reduction in the Phospho-p38 and p38 Expression Levels in the SVZ and SGZ

(A–D) Representative confocal images of the SVZ (A and B) and SGZ (C and D) from 3- and 77-week-old mice, immunostained with antibodies raised against markers for NSCs (SOX2⁺ [magenta]/GFAP⁺ [cyan] cells) and P-p38 (green).

(E–H) Representative confocal images of the SVZ (E and F) and SGZ (G and H) from 8- and 28-week-old mice, immunostained with antibodies raised against markers for TACs (ASCL1⁺ cells [magenta] and P-p38 [green]).

(I) Percentages of phospho-p38 (P-p38)-positive cells in SOX2⁺/GFAP⁺ NSCs in the SVZ (red line) and SGZ (blue line), which decreased with aging.

(J) Percentages of phospho-p38 (P-p38)-positive cells in ASCL1⁺ TACs in the SVZ (red line) and SGZ (blue line), which also decreased with aging.

Values in the line graphs represent the mean ± SD (n = 3 mice). Nuclei were stained with Hoechst (gray). LV, lateral ventricle. Scale bars, 50 μm.