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. 2017 Sep 16;34(3):151–158. doi: 10.5511/plantbiotechnology.17.0727a

Figure 3. Transient effector–reporter analysis of GATA4 transcriptional activity. (A) Schematic representation of the constructs used in transient assay in Arabidopsis leaf protoplasts. The reporter construct contains firefly luciferase (LUC) driven by the promoter containing 5×Gal4 DNA-binding sequence (GAL4BS) and a TATA sequence. Each effector construct contains a Gal4 DNA-binding domain (GALDB) and TMV Omega leader sequence (Ω) driven by the CaMV 35 S promoter (p35S). The effector construct with the SRDX-fused Gal4 DNA-binding domain (GALDB-SRDX) was used as a positive control for repression. (B) Relative LUC reporter activity when each effector was co-transfected into leaf protoplasts. Values are means±SD of six technical replicates. Double asterisks indicate significant difference at p< 0.001 in Dunnett’s test when compared with negative control (GAL4DB).

Figure 3. Transient effector–reporter analysis of GATA4 transcriptional activity. (A) Schematic representation of the constructs used in transient assay in Arabidopsis leaf protoplasts. The reporter construct contains firefly luciferase (LUC) driven by the promoter containing 5×Gal4 DNA-binding sequence (GAL4BS) and a TATA sequence. Each effector construct contains a Gal4 DNA-binding domain (GALDB) and TMV Omega leader sequence (Ω) driven by the CaMV 35 S promoter (p35S). The effector construct with the SRDX-fused Gal4 DNA-binding domain (GALDB-SRDX) was used as a positive control for repression. (B) Relative LUC reporter activity when each effector was co-transfected into leaf protoplasts. Values are means±SD of six technical replicates. Double asterisks indicate significant difference at p< 0.001 in Dunnett’s test when compared with negative control (GAL4DB).