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. 2017 Sep 16;34(3):151–158. doi: 10.5511/plantbiotechnology.17.0727a

Figure 6. Expression of nitrogen assimilation-related genes. Gene expression in shoots (A) and roots (B) of 13 DAS plants grown under different nitrogen conditions, measured by qRT-PCR, and all expression levels were normalized to that of PP2AA3 (At1g3320), reference gene. (A, B) Relative expression of GLN1;1, GLN1;2, GLN1;3, and GLN1;4. (C, D) Relative expression of NIA1 (C) and ASN1 (D) in shoots and roots. Values are means±SD of four independent biological replicates. Single and double asterisks indicate significant differences at p< 0.05 and 0.01, respectively, in t-test when compared to wild type grown in each nitrogen condition.

Figure 6. Expression of nitrogen assimilation-related genes. Gene expression in shoots (A) and roots (B) of 13 DAS plants grown under different nitrogen conditions, measured by qRT-PCR, and all expression levels were normalized to that of PP2AA3 (At1g3320), reference gene. (A, B) Relative expression of GLN1;1, GLN1;2, GLN1;3, and GLN1;4. (C, D) Relative expression of NIA1 (C) and ASN1 (D) in shoots and roots. Values are means±SD of four independent biological replicates. Single and double asterisks indicate significant differences at p< 0.05 and 0.01, respectively, in t-test when compared to wild type grown in each nitrogen condition.