Table 3.
Multiple reaction monitoring transitions, retention time (RT), declustering potential (DP), collision energy (CE), limit of detection (LOD) and limit of quantification (LOQ) for the phytohormones analyzed in method HPLC-2 (6500QTRAP).
| Compound | MRM transition | RT (min) | DP (V) | CE (V) | LODa (pg ⋅ g-1) | LOQa (pg ⋅ g-1) | Linear range (ng ⋅ mL-1) | RSD (%, 0.4 ng ⋅ mL-1)b | RSD (%, 40 ng ⋅ mL-1)b |
|---|---|---|---|---|---|---|---|---|---|
| GA1 | 347.0/259.0 | 2.6 | -65 | -26 | 0.4 | 1.2 | 0.2–200 | 1.9 | 0.9 |
| d2-GA1 | 349.0/261.2 | 2.6 | -140 | -26 | – | – | – | – | – |
| GA3 | 345.0/239.1 | 2.5 | -95 | -20 | 0.2 | 0.5 | 0.2–175 | 1.3 | 0.5 |
| d2-GA3 | 347.0/241.1 | 2.5 | -105 | -20 | – | – | – | – | – |
| GA4 | 331.0/243.2 | 5.6 | -105 | -26 | 0.7 | 2.2 | 0.2–200 | 5.4 | 0.7 |
| d2-GA4 | 333.0/259.0 | 5.6 | -120 | -32 | – | – | – | – | – |
| GA7 | 329.0/223.1 | 5.3 | -80 | -26 | 0.3 | 0.8 | 0.2–175 | 1 | 0.4 |
| d2-GA7 | 331.0/225.1 | 5.3 | -115 | -24 | – | – | – | – | – |
| GA8 | 363.0/275.2 | 1.7 | -105 | -24 | 0.7 | 2.3 | 0.2–90 | 1.5 | 0.4 |
| d2-GA8 | 364.9/277.0 | 1.7 | -125 | -24 | – | – | – | – | – |
| GA12 | 331.0/313.1 | 8.4 | -20 | -38 | 0.4 | 1.2 | 0.2–175 | 2.5 | 0.3 |
| d2-GA12 | 333.0/315.2 | 8.4 | -145 | -36 | – | – | – | – | – |
| GA15 | 329.0/257.0 | 6.7 | -20 | -34 | 0.1 | 0.3 | 0.2–90 | 0.5 | 0.3 |
| d2-GA15 | 331.0/259.1 | 6.7 | -35 | -34 | – | – | – | – | – |
| GA19 | 361.1/273.0 | 4.6 | -60 | -34 | 0.1 | 0.4 | 0.2–200 | 2.5 | 0.7 |
| d2-GA19 | 362.9/275.0 | 4.6 | -115 | -36 | – | – | – | – | – |
| GA20 | 331.1/225.2 | 3.9 | -105 | -34 | 0.5 | 1.8 | 0.2–200 | 2.4 | 0.7 |
| d2-GA20 | 332.9/227.1 | 3.9 | -130 | -34 | – | – | – | – | – |
| GA29 | 347.1/259.1 | 1.9 | -120 | -24 | 0.4 | 1.2 | 0.2–90 | 0.9 | 0.9 |
| d2-GA29 | 348.9/261.2 | 1.9 | -135 | -22 | – | – | – | – | – |
| GA44 | 345.1/272.9 | 4.3 | -130 | -34 | 0.2 | 0.6 | 0.2–175 | 9.3 | 0.9 |
| d2-GA44 | 347.0/275.0 | 4.3 | -120 | -36 | – | – | – | – | – |
| GA51 | 331.1/243.1 | 4.7 | -105 | -22 | 0.5 | 1.6 | 0.2–200 | 2.1 | 0.4 |
| d2-GA51 | 332.9/245.0 | 4.7 | -105 | -24 | – | – | – | – | – |
| GA53 | 347.1/189.0 | 6.0 | -125 | -46 | 0.2 | 0.5 | 0.2–200 | 7.1 | 0.3 |
| d2-GA53 | 349.0/188.9 | 6.0 | -120 | -48 | – | – | – | – | – |
| ABA | 263.1/153.1 | 3.4 | -55 | -16 | 0.1 | 0.3 | 0.2–200 | 1.1 | 0.8 |
| d6-ABA | 269.0/159.0 | 3.4 | -95 | -16 | – | – | – | – | – |
| JA | 209.1/58.9 | 4.1 | -70 | -16 | 0.1 | 0.4 | 0.2–200 | 1.2 | 0.9 |
| d6-JA | 215.0/62.2 | 4.1 | -60 | -18 | – | – | – | – | – |
| IAA | 174.0/129.9 | 2.7 | -95 | -16 | 1.6 | 5.2 | 0.2–200 | 20.0 | 1.6 |
| d5-IAA | 178.9/135.0 | 2.7 | -35 | -16 | – | – | – | – | – |
aLimits of detection (LOD) and quantification (LOQ) were determined in rice samples and are expressed as pg ⋅ g-1 of fresh weight. bValues are average of ten replicates.