Figure 1.

D-stathmin gene, cDNA clones, and protein sequence analyses. (A) Schematic representation of the D-stathmin gene showing the noncoding (white) and coding (gray) exons, as well as the alternatively spliced exon 2′ (black), as identified by comparison with the cDNA clones and with the acceptor/donor splice motifs. Numbers delimiting the exons correspond to nucleotide numeration of clone AAC004639. (B) mRNA-A and -B transcribed from the D-stathmin gene as predicted from both the gene and cDNAs are shown with the structures of the corresponding cDNA clones. (C) Amino acid sequence alignment of D-stathmin-A and -B with mouse (m-) stathmin [identities (∗); homologs (•)]. Exon limits and the previously described domains B, C, D, and E (Maucuer et al., 1990) are indicated. Consensus phosphorylation sites known in m-stathmin are boxed and the corresponding conserved sites in D-stathmins are indicated by arrows. (D) Amino acid identities between D-stathmin and mouse stathmin-related proteins in their aligned B-C-D domains.