Figure 4.

Phosphorylation of D-stathmin-A and -ΔC during the cell cycle. D-stathmin-ΔC-myc and D-stathmin-A-myc overexpressing interphase or mitotic HeLa cell homogenates were analyzed by two-dimensional PAGE-Western blotting with an anti-myc antiserum. Interphase HeLa cell proteins were also treated with phosphatase to identify their phosphorylated and nonphosphorylated forms. The nonphosphorylated (N) or phosphorylated forms of each protein on one, two, or more sites (P1, P2, Pn) are labeled. The likely (arrowheads) and other possible (arrows) phosphorylated residues are indicated on the schematic representation of the D-stathmin constructs.