Figure 8.

RNA interference with D-stathmin dsRNA affects nervous system development. (A–J) Whole mount antibody staining of Drosophila stage 14 embryos, in brown for D-stathmin, and in blue for Futsch with the 22C10 antibody revealing the entire nervous system. (A and C) Ventral views of stage 14 control (A) and dsRNA-injected (C) embryos. The pole cells at the gonads appear clearly labeled for stathmin in the control embryo (A, arrowheads). (B, D, and E) Magnified views of the CNS in control (B) and dsRNA-injected embryos (D and E): fuzzy commissures, defects in the separation of the commissures (arrow), and disruptions of the longitudinal connective (arrowhead) are visible in (D), and failure in the establishment of a commissure (arrowhead) can be seen in E. (G and I) Sagittal views of dsRNA-injected embryos show defects in the peripheral nervous system, visualized with more detail on the magnified views (H and J) showing defects in sensory neuron migration and/or differentiation (arrowheads) compared with the normal embryo (F).