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. 2019 May 23;18(1):443–450. doi: 10.3892/etm.2019.7599

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — TLR2, 4 and 9 mRNA and protein expression levels increase in mouse lung tissue following CLP-induced ALI. WT C57BL/6 male mice were divided into two groups: CLP- and sham-operated groups, and lungs were isolated at 20 h post-surgery. The relative (A) mRNA and (B) protein expression levels of TLR2, 4 and 9 were determined by RT-qPCR and western blot analysis, respectively. Data are presented as the mean ± standard error of the mean (n=5). **P<0.01 vs. sham group. TLR, Τoll-like receptor; CLP, cecal ligation and puncture; ALI, acute lung injury; WT, wild-type; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.