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. 2019 May 23;18(1):475–482. doi: 10.3892/etm.2019.7600

Figure 2.

Figure 2.

Overexpression of miR-485-3p decreases RNF135 expression in glioblastoma cells. (A) Transfection of miR-485-3p mimic increased miR-485-3p levels in U251-MG cells. (B) Overexpression of miR-485-3p decreased RNF135 mRNA levels in U251-MG cells. (C) Representative western blots and quantification demonstrated that overexpression of miR-485-3p decreased RNF135 and p-ERK1/2 protein levels in U251-MG cells. **P<0.01; ***P<0.001, with comparisons indicated by lines. miR, microRNA; RNF135, ring finger protein 135; p-, phosphorylated; NC, negative control.