Table 1.

Gene-specific primers used for the TET2 gene.

Primer	Orientation	Sequence (5′- > 3′)	Position*	Length (bp)
PGL3 Constructs
TET2-Prol-For	Forward	CACTCTTCAAAGAGGAAATATTCC	-633	684
TET2-Prol-Rev	Reverse	TGGGAAATTGACCGAGTTC	+51
TET2-Pro2-For	Forward	CCCAGGTCTCCACTGCGC	+614	411
TET2-Pro2-Rev	Reverse	CTTGCTTCCTTCTGCTTCTC	+1,024
TET2-Pro3-For	Forward	CTCCCTTTCTTCTCCCCTTC	+1,136	479
TET2-Pro3-Rev	Reverse	TCACAAACCCAGACCCAGACC	+1,614
TET2-Pro RT-PCR
TET2-Prol-RT-For	Forward	AGATAGAGACGCGGGCCTCTGAGG	+189
TET2-Pro2-RT-For	Forward	GCAAGGCTGAGGGACGAGAACGAG	+1,082
TET2-Pro3-RT-For	Forward	CAGCCTGGGGAATGTATGTAAGAG	+1,837
TET2-Pro-RT-Rev6	Reverse	CTCITCACTGCTGCITCTGCGAACC	TET2 Exon 6
TET2-Pro-RT-Rev3	Reverse	GGGAGGTGATGGTATCAGGAATGG	TET2 Exon 3

*Relative to the TET-1a transcription start site.