Table III.

Parameters of liquid chromatography-tandem mass spectrometry method for measuring glutamate, aspartate, glutamine and asparagine.

Parameter	Value
Mobile phase	Water containing 0.05% (v/v) formic acid
Column	Phenomenex Kinetex F5 column (4.6×100 mm, 2.6 µm)
Column temperature (°C)	30
Flow rate (ml/min)	0.3
Capillary voltage (kV)	3.5
Drying gas temperature (°C)	350
Drying gas flow (l/min)	10
Nebulizer pressure (psi)	40
MRM transition (m/z)
Glu	148→84
Gln	147→83
Asp	134→88
Asn	133→87
Glu-IS	151→87
Asp-IS	137→91
Dwell (msec)
Glu	100
Gln	100
Asp	100
Asn	100
Glu-IS	100
Asp-IS	100
Fragmentor (V)
Glu	80
Gln	80
Asp	50
Asn	50
Glu-IS	80
Asp-IS	50
CE (eV)
Glu	18
Gln	5
Asp	7
Asn	4
Glu-IS	18
Asp-IS	7

Glu-IS, ²H₃-Glutamate; Asp-IS, ²H₃-Aspartate; CE, Collision Energy