Figure 2.

Anti-proliferative effect of MO frozen extracts. Cell viability. (A, D, G) THP1, (B, E, H) Jurkat cells and (C, F, I) PBMCs from healthy donors after treatment with MOE frozen leaves, fresh seeds and seeds, at a concentration of 0 to 10 mg/ml for 72 h and analysed by trypan blue exclusion test. Control cells (0 mg/ml) were incubated for the same time with an equivalent volume of water. The results are expressed as a fold change of trypan blue positive (black square) or negative cells (white square) with respect to cells at time 0. Data are reported as the mean of three different experiments and of 17 healthy donors' PBMCs ± SD. Symbols indicate significant differences: **P<0.01, ***P<0.001 all treatment vs. untreated cells. ^•••P<0.001 represent the lowest concentration able to significantly reduce or increase cell viability or death, respectively.