Figure 4.

Effect of frozen extract on apoptosis in THP1 cells, Jurkat cells and PBMCs. All cells were treated with frozen preparations of MO (A) leaves, (B) fresh seeds or (C) seeds at a concentration ranging from 0 to 10 mg/ml for 72 h. The data on the Y-axis are expressed as percentage of hypodiploid nuclei detected by propidium iodide staining through flow-cytometry analysis. Data are reported as the mean of three independent experiments and of 17 healthy donors' PBMCs ± SD. Symbols indicate significant differences: *P<0.05, **P<0.01, ***P<0.001 all treatment vs. untreated cells. ⁺⁺P<0.01 represents the lowest concentration able to induce a significant increase in apoptosis in treated cells.