Figure 2.
DCTD is decreased in MDA-MB-231rGEM1 and MDA-MB-231rGEM2 cells compared with parental MDA-MB-231 cells. mRNA expression levels of ABCB1, ABCC10, CMPK1, DCTD, NME1, RRM1 and RRMB1 were detected in MDA-MB-231, MDA-MB-231rGEM1 and MDA-MB-231rGEM2 cells. (A) Compared with the DCTD mRNA expression levels of MDA-MB-231 cells, DCTD was decreased in MDA-MB-231rGEM1 and MDA-MB-231rGEM2 cells. (B) Western blot analysis indicated that DCTD protein expression levels were reduced in MDA-MB-231rGEM1 and MDA-MB-231rGEM2 cells compared with MDA-MB-231 cells. *P<0.05 and **P<0.01 vs. MDA-MB-231 cells. RT-qPCR, reverse transcription-quantitative polymerase chain reaction.