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. 2019 May 10;18(1):312–319. doi: 10.3892/etm.2019.7570

Figure 1.

Figure 1.

TGF-β1 promotes the osteoblastic differentiation of MC3T3-E1 cells. (A) RT-qPCR analysis of the osteogenic-related genes RUNX2, OSX and COL1A1 in MC3T3-E1 cells after treatment with TGF-β1 (4 ng/ml) for 0, 7 and 14 days. (B) Western blotting of RUNX2, COL1A1-and OSX protein in MC3T3-E1 cells cultured with TGF-β1 (4 ng/ml) for 0, 7 and 14 days. (C) ALP activity in MC3T3-E1 cells after treatment with TGF-β1 (4 ng/ml) for 0, 7 and 14 days. NS, no significance change; *P<0.05, **P<0.01 as indicated. TGF, transforming growth factor; RUNX2, runt-related transcription factor 2; OSX, osterix; COL1A1 collagen type I α1 chain; ALP, alkaline phosphatase.